GEMIN5 Rekombinanter Antikörper

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• KD/KO Validated

GEMIN5 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241205G7

Kat-Nr. : 84171-5-PBS

Synonyme

241205G7, Gem associated protein 5, Gem-associated protein 5

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  WB analysis using 84171-5-RR (same clone as 84171-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84171-5-RR (GEMIN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 84171-5-RR (same clone as 84171-5-PBS)

  WB result of GEMIN5 antibody (84171-5-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEMIN5 transfected HEK-293 cells. This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84171-5-RR (same clone as 84171-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84171-5-RR (GEMIN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 84171-5-RR (same clone as 84171-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 84171-5-RR (GEMIN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84171-5-RR (same clone as 84171-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GEMIN5 antibody (84171-5-RR, Clone: 241205G7 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 84171-5-RR (same clone as 84171-5-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GEMIN5 Recombinant antibody (84171-5-RR, Clone:241205G7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84171-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84171-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84171-5-RR against Human GEMIN5 were performed. The affinity constant is 0.619 nM.

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Geprüfte Anwendungen

Produktinformation

84171-5-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA GEMIN5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GEMIN5 fusion protein Ag19220
• Vollständiger Name: gem (nuclear organelle) associated protein 5
• Berechnetes Molekulargewicht: 1508 aa, 169 kDa
• Beobachtetes Molekulargewicht: 168-180 kDa
• GenBank-Zugangsnummer: BC113614
• Gene symbol: GEMIN5
• Gene ID (NCBI): 25929
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.