GEMIN8 Polyklonaler Antikörper

GEMIN8 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15251-1-PBS

Synonyme

Gemin-8, Gemin 8, Gem-associated protein 8, FAM51A1

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  WB analysis using 15251-1-AP (same clone as 15251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15251-1-AP (same clone as 15251-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15251-1-AP (GEMIN8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

  IP result of anti-GEMIN8 (IP:15251-1-AP, 3ug; Detection:15251-1-AP 1:400) with HepG2 cells lysate 1720ug. This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 15251-1-AP (same clone as 15251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15251-1-AP (same clone as 15251-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15251-1-AP (GEMIN8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15251-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15251-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA GEMIN8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GEMIN8 fusion protein Ag6774
• Vollständiger Name: gem (nuclear organelle) associated protein 8
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC003607
• Gene symbol: GEMIN8
• Gene ID (NCBI): 54960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GEMIN8, also named as FAM51A1 and Gemin-8, is associated with the SMN complex and is localized in the cytoplasm and in the nucleus, where it is highly concentrated in Gems. Gemin6, Gemin7, and Unrip form a stable cytoplasmic complex whose association with SMN requires Gemin8. Gemin8 binds directly to SMN and mediates its interaction with the Gemin6/Gemin7 heterodimer. This antibody recognize GEMIN8(28-32kd) only.