GFAP Polyklonaler Antikörper

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• KD/KO Validated

GFAP Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(689)

Rezensionen (11)

Kat-Nr. : 16825-1-AP

Synonyme

glial fibrillary acidic protein

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  WB analysis of U-251 using 16825-1-AP

  WB result of GFAP antibody (16825-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells.

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  WB analysis using 16825-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16825-1-AP (GFAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16825-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16825-1-AP (GFAP antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours.

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  IHC staining of Alzheimer mouse using 16825-1-AP

  Immunohistochemical analysis of paraffin-embedded Hippocampus tissue slide from Alzheimer Mouse Model using 16825-1-AP (GFAP antibody) at dilution of 1:50000 (under 10x lens). Data from NeuroScience Associates, Inc.

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  IHC staining of mouse brain using 16825-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16825-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16825-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 16825-1-AP (GFAP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 16825-1-AP (GFAP antibody) at dilution of 1:1000 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 16825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of A172 using 16825-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A172 cells using GFAP antibody (16825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IF-Fro: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: A172 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 202 publications below
• IHC: See 109 publications below
• IF: See 463 publications below

Produktinformation

16825-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA GFAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Ente, hamster, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFAP fusion protein Ag10423
• Vollständiger Name: glial fibrillary acidic protein
• Berechnetes Molekulargewicht: 432 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC013596
• Gene symbol: GFAP
• Gene ID (NCBI): 2670
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Publikationen

Species	Application	Title
pig	IF	Nature; Injectable ultrasonic sensor for wireless monitoring of intracranial signals; Authors - Hanchuan Tang
mouse	IF	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	IF	Nat Metab; Gut microbiome-derived ammonia modulates stress vulnerability in the host; Authors - Pei Wang
mouse	IF	Nat Metab; Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice; Authors - Wan-Ting Dong
	WB	Nat Commun; Bicarbonate signalling via G protein-coupled receptor regulates ischaemia-reperfusion injury; Authors - Airi Jo-Watanabe
human	IHC	Nat Commun; Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment; Authors - Meilin Xue

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gabriele (Verified Customer) (09-01-2025)

It works very well for IF on hiPSC-derived cortical brain organoid sections (GFAP in yellow and nuclei in blue). The dilution can certainly be increased.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: hiPSC-derived cortical brain organoids

GFAP Antibody Immunofluorescence validation (1:400 dilution) in hiPSC-derived cortical brain organoids (Cat no:16825-1-AP)

FH

Rashmi (Verified Customer) (09-25-2024)

used for western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

FH

Maria-Luisa (Verified Customer) (08-22-2024)

Antigen retrieval with citrate buffer prior to IF staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Cortical organoids

FH

Vikas (Verified Customer) (07-11-2024)

Used for IF, Worked very well, Highly recommended.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Brain tissue

FH

manohar (Verified Customer) (03-06-2024)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293, Neuronal stem cells, Mouse tissue

FH

Badrieh (Verified Customer) (08-02-2022)

this Antibody worked really great with different Recombinant GFAP proteins in ELISA.

• Applications: Other

FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence works well on U251-MG human astrocytoma cell line

• Applications: Immunohistochemistry, Immunofluorescence

FH

Azita (Verified Customer) (05-31-2021)

The human primary cortical cells (DIV28) were subjected to ICC using GFAP antibody (at 1/500 dilution) overnight at 4°C.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Human primary cortical cells

FH

Diane (Verified Customer) (01-03-2020)

I have used this antibody successfully for formalin-fixed paraffin-embedded brain tissues in humans, mouse and rat. I am impressed that there is no non-specific background staining regardless of species. We have used the antibody in a double-staining technique and were able to achieve crisp diagnostic staining for images. The astrocytes were stained using alkaline phosphatase Permanent Red. Antigen retrieval was performed using proteinase K.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: gliotic brain tissue - human

GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in gliotic brain tissue - human (Cat no:16825-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good immuno-fluorescence and western blotting analysis using mouse brain tissues.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

Ryan (Verified Customer) (02-14-2018)

NaCit antigen retrieval ph=6

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Zebrafish retina 5dpf

GFAP Antibody Immunohistochemistry, validation (1:200 dilution) in Zebrafish retina 5dpf (Cat no:16825-1-AP)