GFAP Polyklonaler Antikörper

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• KD/KO Validated

GFAP Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Rezensionen (1)

Kat-Nr. : 23935-1-AP

Synonyme

GFAP

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Galerie der Validierungsdaten

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  WB analysis using 23935-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23935-1-AP (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-251 using 23935-1-AP

  WB result of GFAP antibody (23935-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GFAP transfected U-251 cells.

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  IP experiment of mouse brain using 23935-1-AP

  IP result of anti-GFAP(IP:23935-1-AP, 4ug; Detection:23935-1-AP 1:20000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 23935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain slide using 23935-1-AP (GFAP Antibody) at dilution of 1:50.

• 

  IHC staining of mouse brain using 23935-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain slide using 23935-1-AP (GFAP Antibody) at dilution of 1:50.

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  IHC staining of human gliomas using 23935-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 23935-1-AP (GFAP Antibody) at dilution of 1:50.

• 

  IF Staining of rat brain using 23935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 23935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 23935-1-AP (GFAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 23935-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GFAP antibody (23935-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-251-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 10 publications below

Produktinformation

23935-1-AP bindet in WB, IP, IHC, IF, ELISA GFAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Makake
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFAP fusion protein Ag20853
• Vollständiger Name: glial fibrillary acidic protein
• Berechnetes Molekulargewicht: 432 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC013596
• Gene symbol: GFAP
• Gene ID (NCBI): 2670
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central  nervous system (CNS). GFAP is one of the  main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker.  However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations  present in affected individuals are thought to be gain-of-function. 

• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular  processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various  neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma. 

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916),  which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Publikationen

Species	Application	Title
mouse	IF	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
human	WB	Int J Oncol; Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.; Authors - Wei Jia
human	IF	Eur J Pharmacol; Vitamin D3 analogue calcipotriol inhibits the profibrotic effects of transforming growth factor- β1 on pancreatic stellate cells; Authors - Meifang Zheng
mouse	IF	Sci Rep; Administration of molecular hydrogen during pregnancy improves behavioral abnormalities of offspring in a maternal immune activation model.; Authors - Kenji Imai
rat	WB	Front Pharmacol; Effect of Tanshinone IIA on Gut Microbiome in Diabetes-Induced Cognitive Impairment.; Authors - Yanfang Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Autumn (Verified Customer) (02-08-2024)

Works wonderfully!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: patient derived glioblastoma stem-like cells