GFAP Monoklonaler Antikörper

GFAP Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IP, IHC, IF, Dot blot, ELISA

Konjugation

Unkonjugiert

Publikationen

124

CloneNo.

4B2E10

Rezensionen (8)

Kat-Nr. : 60190-1-Ig

Synonyme

GFAP, glial fibrillary acidic protein, Intermediate filament protein

---

Galerie der Validierungsdaten

• 

  WB analysis using 60190-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60190-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 60190-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60190-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60190-1-Ig (GFAP antibody) at dilution of 1:300000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60190-1-Ig

  IP Result of anti-GFAP (IP:60190-1-Ig, 5ug; Detection:60190-1-Ig 1:1000) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human brain using 60190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human brain using 60190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of mouse brain using 60190-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 60190-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60190-1-Ig (GFAP antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 60190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 60190-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60190-1-Ig (GFAP Antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 60190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 60190-1-Ig (GFAP antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 60190-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GFAP antibody (60190-1-Ig, Clone: 4B2E10 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, Hausschwein-Hirngewebe, humanes Hirngewebe, U-251-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Gliomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

This antibody is not recommended for immunocytofluorescent assays. It is not suitable for frozen sections.

Veröffentlichte Anwendungen

• WB: See 42 publications below
• IHC: See 12 publications below
• IF: See 86 publications below

Produktinformation

60190-1-Ig bindet in WB, IP, IHC, IF, Dot blot, ELISA GFAP und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GFAP fusion protein Ag10452
• Vollständiger Name: glial fibrillary acidic protein
• Berechnetes Molekulargewicht: 432 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-52 kDa
• GenBank-Zugangsnummer: BC013596
• Gene symbol: GFAP
• Gene ID (NCBI): 2670
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Function

GFAP (Glial fibrillary acidic protein) is a type III intermediate filament (IF) protein specific to the central nervous system (CNS). GFAP is one of the main components of the intermediate filament network in astrocytes and has been proposed as playing a role in cell migration, cell motility, maintaining mechanical strength, and in mitosis.

Tissue specificity

GFAP is expressed in central nervous system cells, predominantly in astrocytes. GFAP is commonly used as an astrocyte marker. However, GFAP is also present in peripheral glia and in non-CNS cells, including fibroblasts, chondrocytes, lymphocytes, and liver stellate cells (PMID: 21219963).

Involvement in disease

• Mutations in GFAP lead to Alexander disease (OMIM: 203450), an autosomal dominant CNS disorder. The mutations present in affected individuals are thought to be gain-of-function.
• Upregulation of GFAP is a hallmark of reactive astrocytes, in which GFAP is present in hypertrophic cellular processes. Reactive astrogliosis is present in many neurological disorders, such as stroke, various neurodegenerative diseases (including Alzheimer's and Parkinson's disease), and neurotrauma.

Isoforms

Astrocytes express 10 different isoforms of GFAP that differ in the rod and tail domains (PMID: 25726916), which means that they differ in molecular size. Isoform expression varies during the development and across different subtypes of astrocytes. Not all isoforms are upregulated in reactive astrocytes.

Post-translational modifications

Intermediate filament proteins are regulated by phosphorylation. Six phosphorylation sites have been identified in GFAP protein, at least some of which are reported to control filament assembly (PMID: 21219963).

Cellular localization

GFAP localizes to intermediate filaments and stains well in astrocyte cellular processes.

Publikationen

Species	Application	Title
mouse	WB,IHC	Brain Behav Immun; l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.; Authors - Danqing Xin
human	WB	J Extracell Vesicles; Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.; Authors - Xiaoyu Yu
mouse	WB	Neuron; UBQLN2-HSP70 axis reduces poly-Gly-Ala aggregates and alleviates behavioral defects in the C9ORF72 animal model.; Authors - Kejing Zhang
mouse	IF	Sci Adv; GPR40 modulates epileptic seizure and NMDA receptor function.; Authors - Yong Yang
rat	IF	Small; A Multichannel Flexible Optoelectronic Fiber Device for Distributed Implantable Neurological Stimulation and Monitoring.; Authors - Jingxian Yu
human	WB	EBioMedicine; Inhibition of Nwd1 activity attenuates neuronal hyperexcitability and GluN2B phosphorylation in the hippocampus.; Authors - Qin Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

B (Verified Customer) (01-05-2022)

Very clear and strong signal in WB with a right MW about 50 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

GFAP Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:60190-1-Ig)

FH

Mai (Verified Customer) (01-30-2020)

The antibody seemed to have some background. I will repeat the experiment with different dilutions and conditions to find an optimum dilution for this antibody.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:750
• Cell Tissue Type: Brain Tissue

FH

George (Verified Customer) (08-27-2019)

This gave good staining for activated astrocytes in the 488 channel (1:500 secondary dilution) however there was also significant endogenous background staining which made analysis difficult.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Spinal Cord Mouse Slice Culture

FH

Chintan (Verified Customer) (06-03-2019)

This antibody was used to detect Glial fibrillary acidic protein in primary mouse microglia cells using Western blot. Antibody worked quite well and gave a very specific band near 50 kDa.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: Mouse microglia

GFAP Antibody Western Blot, validation (1:10,000 dilution) in Mouse microglia (Cat no:60190-1-Ig)

FH

Di (Verified Customer) (12-18-2018)

Good antibody!

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Neurons

FH

Lalitha (Verified Customer) (12-13-2018)

Reliable results.

• Applications: Western Blot, Immunohistochemistry,
• Cell Tissue Type: Mice Brain

FH

Chandrakanth (Verified Customer) (12-06-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cerebellum

FH

Emily (Verified Customer) (11-29-2018)

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse brain sections, mouse whole retina, mouse optic nerve sections

Verwandte Produkte

GFAP Polyclonal antibody

16825-1-AP

Vimentin Polyclonal antibody

10366-1-AP

TUBB3-specific/TUJ1 Monoclonal antibody

66375-1-Ig

MAP2 Polyclonal antibody

17490-1-AP

NSE Monoclonal antibody

66150-1-Ig