GFAP Rekombinanter Antikörper

GFAP Rekombinant Antikörper für IF-P

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF-P

Konjugation

CoraLite®532 Fluorescent Dye

CloneNo.

4C6

Kat-Nr. : CL532-81063

Synonyme

4C6, glial fibrillary acidic protein

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  IF Staining of rat brain using CL532-81063

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6 ) at dilution of 1:800. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using CL532-81063

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6 ) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1, red), CoraLite® Plus 488 MBP antibody (CL488-10458, green), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Cyan). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using CL532-81063

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using CoraLite®532 GFAP antibody (CL532-81063, Clone: 4C6 ) at dilution of 1:200, CoraLite®594 NeuN antibody (CL594-66836, Clone: 3A4C1, red), CoraLite® Plus 488 MBP antibody (CL488-10458, green), CoraLite® Plus 647 MAP2 antibody (CL647-84306-3, Clone: 241653E9, Magenta). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL532-81063 bindet in IF-P GFAP und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GFAP fusion protein Ag10423
• Vollständiger Name: glial fibrillary acidic protein
• Berechnetes Molekulargewicht: 432 aa, 50 kDa
• GenBank-Zugangsnummer: BC013596
• Gene symbol: GFAP
• Gene ID (NCBI): 2670
• Konjugation: CoraLite®532 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 537 nm / 560 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GFAP (Glial fibrillary acidic protein ), an intermediate-filament (IF) protein , is specifically expressed in cells of astroglial lineage and is widely used to mark astroglia in the brain. It is also used as a marker for intracranial and intraspinal tumors arising from astrocytes.