GFM1 Polyklonaler Antikörper

GFM1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 14274-1-AP

Synonyme

COXPD1, EF Gmt, EFG1, EFGM, EF-Gmt

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  WB analysis using 14274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14274-1-AP

  IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 14274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 14274-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 14274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14274-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: CTLL-2 cells, HEK-293T-Zellen, HeLa-Zellen, Mausherzgewebe, Rattenherzgewebe, SKOV-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

14274-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA GFM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFM1 fusion protein Ag5616
• Vollständiger Name: G elongation factor, mitochondrial 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC049210
• Gene symbol: GFM1
• Gene ID (NCBI): 85476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]

Publikationen

Species	Application	Title
human	WB	Sci Transl Med; Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.; Authors - Sarah E Calvo
mouse	WB,CoIP	Int J Mol Sci; Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling; Authors - Jana Key
mouse,human	WB	Cells; Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.; Authors - Jana Key
human	WB	Mitochondrion; Toward genotype phenotype correlations in GFM1 mutations.; Authors - Galmiche Louise L

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Miguel (Verified Customer) (03-21-2022)

WB analysis of mouse liver using 14274-1-AP. Mouse liver homogenates in Ripa buffer were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:1000 incubated at 4°C O/N. KI/KO: Compound heterozygous knock-in/knock-out (Gfm1R671C/-) mouse model (1). WT: Wild type control mice (Gfm1+/+). (1) ) Molina-Berenguer M, Vila-Julià F, Pérez-Ramos S, Salcedo-Allende MT, Cámara Y, Torres-Torronteras J, Martí R. Dysfunctional mitochondrial translation and combined oxidative phosphorylation deficiency in a mouse model of hepatoencephalopathy due to Gfm1 mutations. FASEB J. 2022 Jan;36(1):e22091. doi: 10.1096/fj.202100819RRR. PMID: 34919756.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver homogenate

GFM1 Antibody Western Blot validation (1:1000 dilution) in Mouse liver homogenate (Cat no:14274-1-AP)