GFM1 Polyklonaler Antikörper

GFM1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14274-1-PBS

Synonyme

COXPD1, EF Gmt, EFG1, EFGM, EF-Gmt

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  WB analysis using 14274-1-AP (same clone as 14274-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14274-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA GFM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFM1 fusion protein Ag5616
• Vollständiger Name: G elongation factor, mitochondrial 1
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC049210
• Gene symbol: GFM1
• Gene ID (NCBI): 85476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]