GFP tag Monoklonaler Antikörper

GFP tag Monoklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

1E10H7

Kat-Nr. : 66002-1-PBS

Synonyme

eGFP, GFP, CFP, eYFP, GFP-tag

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  WB analysis using 66002-1-Ig (same clone as 66002-1-PBS)

  HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

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  WB analysis of recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

  WB result of eGFP fusion protein with anti-eGFP tag (66002-1-Ig) with GFP fusion protein at varous dilutions. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Recombinant protein using 66002-1-Ig (same clone as 66002-1-PBS)

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

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  IP experiment of Transfected HEK-293T using 66002-1-Ig (same clone as 66002-1-PBS)

  IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:10000) with Transfected HEK-293T cells lysate 400 ug. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (66002-1-Ig, Clone: 1E10H7 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66002-1-PBS bindet in WB, IF/ICC, IP, ELISA GFP tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GFP tag fusion protein Ag2128
• Vollständiger Name: GFP tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: M62653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.