GFP tag Rekombinanter Antikörper

GFP tag Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

aequorea victoria, rekombinanten Protein

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241614B3

Kat-Nr. : 81835-2-RR

Synonyme

241614B3

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  WB analysis of Recombinant protein using 81835-2-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 81835-2-RR

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81835-2-RR (GFP tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected COS-7 using 81835-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected COS-7 cells using GFP tag antibody (81835-2-RR, Clone: 241614B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81835-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81835-2-RR against Aequorea victoria GFP tag were performed. The affinity constant is 0.619 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: Transfected COS-7

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81835-2-RR bindet in WB, IF/ICC, ELISA GFP tag und zeigt Reaktivität mit aequorea victoria, rekombinanten Protein

• Getestete Reaktivität: aequorea victoria, rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GFP tag fusion protein Ag2128
• Vollständiger Name: GFP tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: M62653
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm. When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy. This antibody is generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.