GFP tag Monoklonaler Antikörper

GFP tag Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1E10H7

Kat-Nr. : CL647-66002

Synonyme

eGFP, GFP, CFP, eYFP, GFP-tag

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  IF Staining of Transfected HEK-293 using CL647-66002

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 GFP tag antibody (CL647-66002, Clone: 1E10H7 ) at dilution of 1:200.

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  FC experiment of Transfected HEK-293T using CL647-66002

  1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated GFP tag Monoclonal antibody (CL647-66002, Clone:1E10H7), and 0.4 ug CoraLite® Plus 647 Mouse IgG2a isotype control (11A1B2) (CL647-66360-2, Clone: 11A1B2). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfected HEK-293T cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-66002 bindet in IF/ICC, FC (Intra) GFP tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GFP tag fusion protein Ag2128
• Vollständiger Name: GFP tag
• Berechnetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: M62653
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 647.