GFPT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GFPT1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (1)

Kat-Nr. : 14132-1-AP

Synonyme

GFA, GFAT, GFAT 1, GFAT1, GFAT1m, GFPT, GFPT1

---

Galerie der Validierungsdaten

• 

  WB analysis of human skeletal muscle using 14132-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14132-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 14132-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14132-1-AP

  Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Skelettmuskelgewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

14132-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA GFPT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFPT1 fusion protein Ag5312
• Vollständiger Name: glutamine-fructose-6-phosphate transaminase 1
• Berechnetes Molekulargewicht: 681 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC045641
• Gene symbol: GFPT1
• Gene ID (NCBI): 2673
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)

Publikationen

Species	Application	Title
human	WB	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
		Circ Res; Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.; Authors - Felipe Paredes
human	IF	J Clin Invest; Targeting tumor-intrinsic hexosamine biosynthesis sensitizes pancreatic cancer to anti-PD1 therapy.; Authors - Nikita S Sharma
	WB	Nat Commun; Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection.; Authors - Adela S Oliva Chávez
human	WB	Am J Hum Genet; Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect.; Authors - Senderek Jan J

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Kristian (Verified Customer) (06-07-2021)

Mouse testis were stained for Gfpt1 (green) and DAPI (blue)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse testis

GFPT1 Antibody Immunofluorescence validation (1:200 dilution) in Mouse testis (Cat no:14132-1-AP)