GFRA2 Polyklonaler Antikörper

GFRA2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21973-1-AP

Synonyme

GDNF family receptor alpha 2, GDNF family receptor alpha-2, GDNF receptor alpha 2, GDNF receptor alpha-2, GDNF receptor beta

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  WB analysis using 21973-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21973-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21973-1-AP (GFRA2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 21973-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21973-1-AP (GFRA2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, L02-Zellen, Maushirngewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below

Produktinformation

21973-1-AP bindet in WB, IHC, ELISA GFRA2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GFRA2 fusion protein Ag16893
• Vollständiger Name: GDNF family receptor alpha 2
• Berechnetes Molekulargewicht: 464 aa, 52 kDa
• Beobachtetes Molekulargewicht: 34-40 kDa, 50-55 kDa
• GenBank-Zugangsnummer: BC041688
• Gene symbol: GFRA2
• Gene ID (NCBI): 2675
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GFRA2, also named as GDNFRB, RETL2 and TRNR2, belongs to the GDNFR family. It is a receptor for neurturin. GFRA2 mediates the NRTN-induced autophosphorylation and activation of the RET receptor. It is a potent survival factor for central dopaminergic neurons, motor neurons, and several other populations of neurons in the central and peripheral nervous systems. The GDNF signal is mediated by a complex of the receptor tyrosine kinase RET and a glycosylphosphatidylinositol (GPI)-linked protein, GDNFRA. GFRA2 has three isoforms with MW 34-36 kDa, 39-41 kDa and 50-55 kDa. This antibody recognizes all the three isoforms.

Publikationen

Species	Application	Title
human	IHC	Cancer Lett; GFRα2 prompts cell growth and chemoresistance through down-regulating tumor suppressor gene PTEN via Mir-17-5p in pancreatic cancer.; Authors - Jiangning Gu
rat	IHC	Sci Rep; Hypoxia adaptation mechanism in rats' peripheral auditory system in high altitude migration: a time series transcriptome analysis; Authors - Luoying Wang
rat	WB	Biotechnol Appl Biochem; Ellagic acid supports neuron by regulating astroglia Nrf2.; Authors - Guo-Qing Wang