GGCX Polyklonaler Antikörper

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GGCX Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16209-1-PBS

Synonyme

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• 

  WB analysis of MCF-7 using 16209-1-AP (same clone as 16209-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16209-1-AP (same clone as 16209-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 16209-1-AP (same clone as 16209-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16209-1-AP (same clone as 16209-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 16209-1-AP (same clone as 16209-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16209-1-AP (same clone as 16209-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16209-1-AP (same clone as 16209-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 16209-1-AP (same clone as 16209-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 16209-1-AP (same clone as 16209-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 16209-1-AP (same clone as 16209-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 16209-1-AP (same clone as 16209-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 16209-1-AP (same clone as 16209-1-PBS)

  IP result of anti-GGCX (IP:16209-1-AP, 4ug; Detection:16209-1-AP 1:2000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16209-1-AP (same clone as 16209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 16209-1-AP (same clone as 16209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16209-1-PBS bindet in WB, IHC, IP, Indirect ELISA GGCX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GGCX fusion protein Ag9185
• Vollständiger Name: gamma-glutamyl carboxylase
• Berechnetes Molekulargewicht: 758 aa, 88 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC013979
• Gene symbol: GGCX
• Gene ID (NCBI): 2677
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).