GGGGS Linker Rekombinanter Antikörper

GGGGS Linker Rekombinant Antikörper für WB, IHC, IF/ICC, FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IHC, IF/ICC, FC

Konjugation

Unkonjugiert

CloneNo.

242306C8

Kat-Nr. : 98262-1-PBS

Synonyme

G4S, G4S link, G4S Linker, GGGGS, GS Linker

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  WB analysis using 98262-1-RR (same clone as 98262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 98262-1-RR (GGGGS Linker antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  IHC staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Transfected HEK-293T cells slide using 98262-1-RR (GGGGS Linker antibody) at dilution of 1:80000 (under 40x lens). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293T cells using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected CHO-K1 using 98262-1-RR (same clone as 98262-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected CHO-K1 using GGGGS Linker antibody (98262-1-RR, Clone: 242306C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone:242306C8) and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005) (red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98262-1-RR (same clone as 98262-1-PBS)

  1x10^6 Jurkat cells or NK92 cells or Raji cells were surface stained with 0.25 ug Anti G4S Linker Rabbit RecAb (98262-1-RR, Clone: 242306C8) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed This data was developed using the same antibody clone with 98262-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98262-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98262-1-RR against scFv (single chain fragment variable) format with variable regions of heavy (VH) and light (VL) chains joined together by G4S linker were performed. The affinity constant is 3.87 nM.

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Geprüfte Anwendungen

Produktinformation

98262-1-PBS bindet in WB, IHC, IF/ICC, FC GGGGS Linker und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GGGGS Linker
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404). This antibody, raised against a synthetic peptide (GGGGSGGGGSGGGGS), can recognize G4S, G4S*2, G4S*3, G4S*4, and G4S*5.