GGGGS Linker Rekombinanter Antikörper

GGGGS Linker Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

FC

Konjugation

Biotin

CloneNo.

242306C8

Kat-Nr. : Biotin-98262

Synonyme

G4S, G4S link, G4S Linker, GGGGS, GS Linker

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  FC experiment of Transfected HEK-293T using Biotin-98262

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Biotin Plus Anti-GGGGS Linker Rabbit RecAb (Biotin-98262, Clone: 242306C8) and CoraLite®488-conjugated streptavidin. Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using Biotin-98262

  1x10^6 HEK-293T cells or anti-CD19 (FMC63) CAR with CD28 or CD8 hinge region and transmembrane region transfected HEK-293T cells were surface stained with 0.25 ug Biotin Plus Anti-GGGGS Linker Rabbit RecAb (Biotin-98262, Clone:242306C8) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-98262 bindet in FC GGGGS Linker und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GGGGS Linker
• Gene ID (NCBI): 42
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

As a crucial element in the design of recombinant fusion proteins, linkers play an increasingly vital role in the construction of stable, bioactive fusion proteins (PMID: 23026637). GGGGS linker (G4S linker) is a flexible linker made of 4 glycine repeats followed by a serine amino acid (PMID:3045807). Due to its flexibility and resistance to proteases, GGGGS and its repeats are commonly used when engineering a protein, particularly in the construction of single-chain Fv (ScFv) domains expressed on the surfaces of CAR-T cells (PMID:23581628; 36874404).