GGT1 Rekombinanter Antikörper

GGT1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230428B5

Kat-Nr. : 83304-1-PBS

Synonyme

230428B5, CD224, D22S672, D22S732, GGT

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  WB analysis using 83304-1-RR (same clone as 83304-1-PBS)

  Human kidney tissue were subjected to SDS PAGE followed by western blot with 83304-1-RR (GGT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83304-1-RR (same clone as 83304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83304-1-RR (GGT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83304-1-RR (same clone as 83304-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83304-1-RR (GGT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

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  IHC staining of Human Kidney(renal cell) Cancer using 83304-1-PBS

  Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 83304-1-PBS (GGT1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83304-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 83304-1-PBS against Human GGT1 was performed. The affinity constant is 1.86 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Human Kidney(renal cell) Cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83304-1-PBS bindet in WB, IHC, Indirect ELISA GGT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GGT1 fusion protein Ag34209
• Vollständiger Name: gamma-glutamyltransferase 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: NM_013430
• Gene symbol: GGT1
• Gene ID (NCBI): 2678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GGT1 is a transpeptidase whose activity increases the availability of amino acids, primarily cysteine, for intracellular GSH synthesis. GGT1 also plays a critical role in maintaining GSH homeostasis and defense against oxidative stress. In liver and obstructive biliary diseases, circulating GGT1 activity has widely been used for diagnosis purposes, as well as an indicator of alcohol consumption. (PMID: 31151999)