Growth Hormone Polyklonaler Antikörper

Growth Hormone Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 55243-1-AP

Synonyme

GH, GH1, growth hormone 1, GH-N, GH 1

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  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 55243-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 55243-1-AP (GH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pituitary using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pituitary adenoma using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pituitary adenoma using 55243-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary adenoma tissue slide using 55243-1-AP (GH1 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Hypophysengewebe, humanes Hypophysenadenom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 3 publications below

Produktinformation

55243-1-AP bindet in WB, IHC, IF, ELISA Growth Hormone und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GH1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_000515
• Gene symbol: GH1
• Gene ID (NCBI): 2688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues. This antibody has no cross reaction to CSH1.

Publikationen

Species	Application	Title
rat	IF	Metabolism; PMID: 31666193; Authors - Jian Mao
mouse	WB	Psychoneuroendocrinology; Transcriptomics study of hippocampus in mice exposed to heat stress; Authors - Bin Li
mouse	WB,IF	Biochem Biophys Res Commun; Elongator regulates the melanocortin satiety pathway.; Authors - Joseph Walters
rat	WB	Toxicol Lett; The disrupting effect of chlormequat chloride on growth hormone is associated with pregnancy; Authors - Zongzhen Wu
	IF	Front Sleep; Regulation of dendritic spines in the amygdala following sleep deprivation; Authors - Lindsay Rexrode
human	WB	Talanta; What does one-dimensional gel electrophoresis-based western blotting data really mean in the reality of proteoforms; Authors - Lamei Yang