Growth Hormone Rekombinanter Antikörper

Growth Hormone Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4G8

Kat-Nr. : 82813-2-RR

Synonyme

GH, GH1, 4G8, GH 1, GH-N

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  WB analysis using 82813-2-RR

  Human placenta tissue was subjected to SDS PAGE followed by western blot with 82813-2-RR (GH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82813-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82813-2-RR (Growth Hormone antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 82813-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 82813-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 82813-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (Growth Hormone antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82813-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82813-2-RR against Human GH1 were performed. The affinity constant is 0.76 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva, humanes Plazenta-Gewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:1250-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82813-2-RR bindet in WB, IHC, ELISA Growth Hormone und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Growth Hormone fusion protein Eg0383
• Vollständiger Name: GH1
• Berechnetes Molekulargewicht: 217 aa, 25 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC075012
• Gene symbol: GH1
• Gene ID (NCBI): 2688
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.