GHITM Polyklonaler Antikörper

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GHITM Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16296-1-PBS

Synonyme

Dermal papilla-derived protein 2, DERP2, Growth hormone-inducible transmembrane protein, MICS1, My021

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  WB analysis of mouse liver using 16296-1-AP (same clone as 16296-1-PBS)

  mouse liver mitochondria tissue were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16296-1-AP (same clone as 16296-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16296-1-AP (same clone as 16296-1-PBS)

  WB results of GHITM antibody (16296-1-AP) with siRNA sample. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16296-1-AP (same clone as 16296-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 16296-1-AP (same clone as 16296-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16296-1-AP (same clone as 16296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16296-1-AP (GHITM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16296-1-AP (same clone as 16296-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16296-1-AP (GHITM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16296-1-AP (same clone as 16296-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GHITM antibody (16296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16296-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GHITM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GHITM fusion protein Ag9482
• Vollständiger Name: growth hormone inducible transmembrane protein
• Berechnetes Molekulargewicht: 345 aa, 37 kDa
• Beobachtetes Molekulargewicht: 42 kDa, 25-27 kDa
• GenBank-Zugangsnummer: BC010354
• Gene symbol: GHITM
• Gene ID (NCBI): 27069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein which localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa. The apparent molecular weight has been reported to be 42 kDa, the increased size in the protein may be due to post-translational modifications (PMID: 11416014; 16412389). GHITM can be cleaved into smaller forms of 23-27 kDa (PMID: 16412389; 18417609).