GHITM Rekombinanter Antikörper

GHITM Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240541D3

Kat-Nr. : 83548-3-PBS

Synonyme

240541D3, Dermal papilla-derived protein 2, DERP2, Growth hormone-inducible transmembrane protein, MICS1

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  WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83548-3-RR (same clone as 83548-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug GHITM Recombinant antibody (83548-3-RR, Clone:240541D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00524-1

  Cytometric bead array standard curve of MP00524-1, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-2-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00524-2

  Cytometric bead array standard curve of MP00524-2, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-1-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83548-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM.

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Geprüfte Anwendungen

Produktinformation

83548-3-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA GHITM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GHITM fusion protein Ag35170
• Vollständiger Name: growth hormone inducible transmembrane protein
• Berechnetes Molekulargewicht: 345 aa, 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC010354
• Gene symbol: GHITM
• Gene ID (NCBI): 27069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein that localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa.