VISTA Polyklonaler Antikörper

VISTA Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 24849-1-AP

Synonyme

C10orf54, B7-H5, Sisp-1, SISP1, PP2135

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  WB analysis using 24849-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24849-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24849-1-AP (VISTA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24849-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24849-1-AP (VISTA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 24849-1-AP (VISTA rabbit polyclonal antibody) at dilution of 1:100 and 60181-1-Ig (CD3 mouse monoclonal antibody), further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 24849-1-AP and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60181-1-Ig.

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  IF Staining of human placenta using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 24849-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (24849-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Kolonkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe, humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

24849-1-AP bindet in WB, IHC, IF-P, ELISA VISTA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VISTA fusion protein Ag20600
• Vollständiger Name: chromosome 10 open reading frame 54
• Berechnetes Molekulargewicht: 311 aa, 34 kDa
• Beobachtetes Molekulargewicht: 45-60 kDa
• GenBank-Zugangsnummer: BC020568
• Gene symbol: VISTA
• Gene ID (NCBI): 64115
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion.

Publikationen

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	IF	Front Cell Dev Biol; Identify the Prognostic and Immune Profile of VSIR in the Tumor Microenvironment: A Pan-Cancer Analysis.; Authors - Yuanyuan Liu