GILZ Polyklonaler Antikörper

GILZ Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12352-1-PBS

Synonyme

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  WB analysis of mouse brain using 12352-1-AP (same clone as 12352-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12352-1-AP (same clone as 12352-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12352-1-AP (same clone as 12352-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12352-1-AP (GILZ antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12352-1-AP (same clone as 12352-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12352-1-AP (GILZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 12352-1-AP (same clone as 12352-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using GILZ antibody (12352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12352-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12352-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GILZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GILZ fusion protein Ag3015
• Vollständiger Name: TSC22 domain family, member 3
• Berechnetes Molekulargewicht: 134 aa, 15 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC018148
• Gene symbol: GILZ
• Gene ID (NCBI): 1831
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GILZ, also known as TSC22D3 and DSIPI, is a glucocorticoid responsive protein and is thought to mediate some of the anti-inflammatory effects of glucocorticoid receptors (GRs). GILZ is mainly localized in the cytoplasm. It is an anti-inflammatory molecule that could mediate the immunomodulatory activities of GCs and also plays a role in cell differentiation in various cells and tissues (PMID: 35011684).