GIRK2 Rekombinanter Antikörper

GIRK2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250605D1

Kat-Nr. : 86016-1-RR

Synonyme

KCNJ6, BIR1, G protein-activated inward rectifier potassium channel 2, GIRK 2, GIRK-2

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  WB analysis using 86016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86016-1-RR (KCNJ6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86016-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86016-1-RR (KCNJ6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86016-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86016-1-RR against Human GIRK2 were performed. The affinity constant is 0.372 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MG-63 cells, Maushirngewebe, NIH/3T3-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86016-1-RR bindet in WB, ELISA GIRK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GIRK2 fusion protein Ag16344
• Vollständiger Name: potassium inwardly-rectifying channel, subfamily J, member 6
• Berechnetes Molekulargewicht: 423 aa, 48 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC101547
• Gene symbol: GIRK2
• Gene ID (NCBI): 3763
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GIRK2 (also known as Kir3.2), encoded by KCNJ6 gene, is a member of the G protein-coupled inwardly-rectifying potassium channel (GIRK, Kir3) family of inward rectifier potassium channels. GIRK channels are activated following stimulation of G protein-coupled receptors (PMID: 26422984). They play important roles in regulating cellular excitabilities in the heart and brain (PMID: 31043612). Mutations in KCNJ6 gene are associated with Keppen-Lubinsky Syndrome (PMID: 25620207).