GITR/TNFRSF18 Rekombinanter Antikörper

GITR/TNFRSF18 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

250666B12

Kat-Nr. : 98466-3-PBS

Synonyme

CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein

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  FC experiment of mouse splenocytes using 98466-3-RR (same clone as 98466-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse GITR/TNFRSF18 Rabbit RecAb (98466-3-RR, Clone: 250666B12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD25/IL-2RA (PC61.5) (APC-65137, Clone: PC61.5). CD4+ cells were pre-gated for analysis. Cells were not fixed. This data was developed using the same antibody clone with 98466-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98466-3-RR (same clone as 98466-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse GITR/TNFRSF18 Rabbit RecAb (98466-3-RR, Clone:250666B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). CD4+ cells were pre-gated for analysis. Cells were not fixed. This data was developed using the same antibody clone with 98466-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98466-3-PBS bindet in FC GITR/TNFRSF18 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GITR/TNFRSF18 fusion protein Eg3695
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 18
• Berechnetes Molekulargewicht: 25kDa
• GenBank-Zugangsnummer: NM_009400.2
• Gene symbol: CD357
• Gene ID (NCBI): 21936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).