GITR/TNFRSF18 Rekombinanter Antikörper

GITR/TNFRSF18 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

250666B12

Kat-Nr. : 98466-3-RR

Synonyme

CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein

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  FC experiment of mouse splenocytes using 98466-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse GITR/TNFRSF18 Rabbit RecAb (98466-3-RR, Clone: 250666B12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD25/IL-2RA (PC61.5) (APC-65137, Clone: PC61.5). CD4+ cells were pre-gated for analysis. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98466-3-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse GITR/TNFRSF18 Rabbit RecAb (98466-3-RR, Clone:250666B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). CD4+ cells were pre-gated for analysis. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

98466-3-RR bindet in IF, FC GITR/TNFRSF18 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GITR/TNFRSF18 fusion protein Eg3695
• Vollständiger Name: tumor necrosis factor receptor superfamily, member 18
• Berechnetes Molekulargewicht: 25kDa
• GenBank-Zugangsnummer: NM_009400.2
• Gene symbol: CD357
• Gene ID (NCBI): 21936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).

Publikationen

Species	Application	Title
mouse	IF	Adv Healthc Mater; Engineered Hollow Nanocomplex Combining Photothermal and Antioxidant Strategies for Targeted Tregs Depletion and Potent Immune Activation in Tumor Immunotherapy; Authors - Qi Sun