Connexin-32 Polyklonaler Antikörper

Connexin-32 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10450-1-AP

Synonyme

CMTX, CMTX1, Connexin-32, CX32, Gap junction beta 1 protein, GJB1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 10450-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10450-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10450-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10450-1-AP

  IP Result of anti-Connexin-32 (IP:10450-1-AP, 4ug; Detection:10450-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (GJB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (GJB1 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A375-Zellen, humanes Lebergewebe, Maushautgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

10450-1-AP bindet in WB, IP, IHC, IF, ELISA Connexin-32 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Connexin-32 fusion protein Ag0659
• Vollständiger Name: gap junction protein, beta 1, 32kDa
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC002805
• Gene symbol: GJB1
• Gene ID (NCBI): 2705
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Connexin-32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-32 is the major gap junction protein of liver, and the expression is also found in brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin-32 plays an important role in peripheral nerve. Defects in Connexin-32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. (PMID: 8266101; 8613761; 8608591; 19265674)

Publikationen

Species	Application	Title
human	WB	Chemosphere; Organophosphate flame retardant TDCPP: A risk factor for renal cancer?; Authors - Xuan Zhou
human	WB	World J Gastroenterol; PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis.; Authors - Xiao-Ming Liu
mouse	WB	Brain Res Bull; Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice.; Authors - Chen Men
human	IHC	J Biomed Biotechnol; Ischemia alters the expression of connexins in the aged human brain.; Authors - Nakase Taizen T
human	IHC, IF	Histopathology; Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features.; Authors - Wen-Qun Zhong
human	WB	Oncol Lett; Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.; Authors - Lihua Huang

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