Gastrokine 1 Polyklonaler Antikörper

Gastrokine 1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 19344-1-AP

Synonyme

18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11

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  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 19344-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 19344-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 19344-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach using 19344-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 19344-1-AP (Gastrokine 1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Magengewebe, Maus-Magengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

19344-1-AP bindet in WB, IF, IHC, ELISA Gastrokine 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Gastrokine 1 fusion protein Ag6091
• Vollständiger Name: gastrokine 1
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 20-22 kDa
• GenBank-Zugangsnummer: BC059778
• Gene symbol: Gastrokine 1
• Gene ID (NCBI): 56287
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gastrokine 1 (GKN1), a stomach-specific protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene has been localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. GKN1 is expressed only in normal human stomach, but is absent from gastric adenocarcinomas, gastro-esophageal adenocarcinoma cell lines, and other normal and tumor gastro-intestinal tissues. GKN1may play an important role in normal gastric function and may be a gastric tumour suppressor.

Publikationen

Species	Application	Title
mouse	WB	Sci Rep; Gastrokine-1, an anti-amyloidogenic protein secreted by the stomach, regulates diet-induced obesity.; Authors - Anne-Marie C Overstreet
human	WB,IHC	Helicobacter; Helicobacter pylori inhibits GKN1 expression via the CagA/p-ERK/AUF1 pathway.; Authors - Yanlei Guo
mouse	WB,IF,ELISA	Int J Biol Sci; IL-33 Accelerates Chronic Atrophic Gastritis through AMPK-ULK1 Axis Mediated Autolysosomal Degradation of GKN1; Authors - Kewei Liu
mouse	WB	Cell Mol Gastroenterol Hepatol; Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia; Authors - Pawan Puri