Beta Galactosidase Polyklonaler Antikörper

Beta Galactosidase Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(45)

Rezensionen (4)

Kat-Nr. : 15518-1-AP

Synonyme

GLB1, Acid beta-galactosidase, Beta-galactosidase, EBP, EC:3.2.1.23

---

• 

  WB analysis of SH-SY5Y using 15518-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 15518-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15518-1-AP (GLB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta Galactosidase antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15518-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15518-1-AP (Beta galactosidase Antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human prostate cancer using 15518-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human prostate cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human liver cancer using 15518-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver cancer tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 15518-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15518-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Beta Galactosidase antibody (15518-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Gliomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe, Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 14 publications below
• IF: See 10 publications below
• IP: See 1 publications below

Produktinformation

15518-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA Beta Galactosidase und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Beta Galactosidase fusion protein Ag7792
• Vollständiger Name: galactosidase, beta 1
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC007493
• Gene symbol: GLB1/Beta-galactosidase
• Gene ID (NCBI): 2720
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the molecular weight of β-galactosidase (GLB1)?

The molecular weight of GLB1 is 76 kD, although this protein is known to have multiple isoforms with lower 

molecular weight values.

What is the function of GLB1?

The common name of β-galactosidase is lactase. This enzyme is responsible for catalyzing the hydrolysis of 

lactose to monosaccharide glucose and galactose. GLB1 also catalyzes hydrolysis of polymeric D-galactosyl 

residues (PMID: 23526073, doi: 10.1088/1757-899x/263/2/022046).

How is GLB1 used in our food?

GLB1 isolated from bacteria, fungi, yeast, plants, and other sources has been applied to the manufacturing 

of lactose-hydrolyzed products (PMID: 28500401).

How is GLB1 part of a positive feedback loop?

GLB1 transgalactosylates lactose, resulting in allolactose. Allolactose is the inducer for the lac operon and 

binds to the lac repressor, prompting GLB1 synthesis (PMIDs: 23526073, 28500401, 26394634).

What organisms express GLB1?

GLB1 is ubiquitous in nature and is produced across kingdoms in plants, microorganisms, and animals. 

Properties, such as optimal pH range and temperature preference, vary between species; fungal GLB1 

prefers acidic conditions, while yeast and bacteria have optimal conditions at more neutral pH values

(PMID: 28500401).

How is GLB1 utilized in transgenic animals?

Bacterial GLB1 is a widely used reporter gene in transgenic or knockout animals. The lacZ gene of E. coli 

has been used to identify cis-acting DNA elements that play a key role for the regulation of gene expression 

in mice. The lacZ gene can be incorporated into an endogenous gene, and it is frequently a used to indicate 

the success of Cre-loxP recombination events, which allows temporal control of lacZ expression. Mice a 

part of the Rosa26R line that also express Cre ubiquitously express Cre-inducible lacZ (PMID: 26394634).

Publikationen

Species	Application	Title
human		Cell Death Differ; ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity; Authors - Feng Xu
	IHC	Environ Int; HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity; Authors - Xiaowei Cong
human	IF	Aging Cell; Galactose-modified duocarmycin prodrugs as senolytics.; Authors - Ana Guerrero
mouse	WB	J Cachexia Sarcopenia Muscle; Sarcopenia is attenuated by TRB3 knockout in aging mice via the alleviation of atrophy and fibrosis of skeletal muscles.; Authors - Guo-Kai Shang
human	IHC	Theranostics; Herb-sourced emodin inhibits angiogenesis of breast cancer by targeting VEGFA transcription.; Authors - Gengyi Zou
mouse	WB,IF	Cancer Lett; Legumain-deficient macrophages promote senescence of tumor cells by sustaining JAK1/STAT1 activation.; Authors - Long Shen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Maelle (Verified Customer) (12-19-2024)

Functional

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1.50eme
• Cell Tissue Type: Healthy Colon

FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Work well in WB

• Applications: Western Blot
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: Mouse Heart Tissue

Beta Galactosidase Antibody Western Blot validation (1/500 dilution) in Mouse Heart Tissue (Cat no:15518-1-AP)

FH

Lin (Verified Customer) (02-24-2022)

It is good for WB.

• Applications: Western Blot

FH

Lin (Verified Customer) (06-04-2021)

It works for skin IHC.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human skin