GLI1 Polyklonaler Antikörper

GLI1 Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 25733-1-AP

Synonyme

GLI, GLI family zinc finger 1, GLI1, Glioma associated oncogene, Oncogene GLI, Zinc finger protein GLI1

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Galerie der Validierungsdaten

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  IHC staining of human gliomas using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 25733-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25733-1-AP (GLI1 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

Empfohlene Verdünnung

Anwendung	Verdünnung
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

25733-1-AP bindet in WB, IF, ELISA GLI1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLI1 fusion protein Ag22962
• Vollständiger Name: GLI family zinc finger 1
• Berechnetes Molekulargewicht: 1106 aa, 118 kDa
• GenBank-Zugangsnummer: BC013000
• Gene symbol: GLI1
• Gene ID (NCBI): 2735
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Lab Chip; Paper-based microreactor array for rapid screening of cell signaling cascades.; Authors - Chia-Hao Huang
human	IF	Oncotarget; Investigating the role of Hedgehog/GLI1 signaling in glioblastoma cell response to temozolomide.; Authors - Jilian R Melamed
human	WB	Hum Cell; Retraction Note: Long noncoding RNA LINC00460 promotes the progression of cervical cancer via regulation of the miR-361-3p/Gli1 axis; Authors - Fan Li
human	WB	Oncol Rep; Combined inhibition of sonic Hedgehog signaling and histone deacetylase is an effective treatment for liver cancer.; Authors - Jingmin Li
human	WB	Am J Transl Res; Involvement of hedgehog signaling in all-trans retinoic acid-mediated suppression of colon cancer; Authors - Yu Xu
		Biomed Environ Sci; Effect of Smo SiRNA-mediated Hedgehog Signaling Pathway Inhibition on Palatal Fusion.; Authors - Qian Zhou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (12-20-2018)

Cells were fixed in 4% paraformaldehyde and stained overnight at 4C. Cells were counterstained with phalliodin and DAPI. Staining is mix of weak nuclear and punctate cytosolic.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: U87 Human Glioblastoma

GLI1 Antibody Immunofluorescence, validation (1:500 dilution) in U87 Human Glioblastoma (Cat no:25733-1-AP)