GLI1 Monoklonaler Antikörper

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• KD/KO Validated

GLI1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D2B2

Kat-Nr. : 66905-1-PBS

Synonyme

1D2B2, GLI, GLI family zinc finger 1, Glioma associated oncogene, Glioma-associated oncogene

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  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 66905-1-Ig (same clone as 66905-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66905-1-Ig (same clone as 66905-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66905-1-Ig (GLI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66905-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66905-1-PBS bindet in WB, ELISA GLI1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GLI1 fusion protein Ag27832
• Vollständiger Name: GLI family zinc finger 1
• Berechnetes Molekulargewicht: 1106 aa, 118 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC013000
• Gene symbol: GLI1
• Gene ID (NCBI): 2735
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLI1, also known as Glioma-associated oncogene, is a 1106 amino acid protein, which belongs to the GLI C2H2-type zinc-finger protein family. GLI1 is detected in testis and Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. GLI1 is tethered in the cytoplasm by binding to SUFU (PubMed:10806483). GLI1 is activated and translocated to the nucleus promoted by interaction with STK36 (PubMed:10806483). Phosphorylation of GLI1 by ULK3 may promote nuclear localization (PubMed:19878745). GLI1 as a transcriptional activator may regulate the transcription of specific genes during normal development (PubMed:19706761). GLI1 plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract and Mediates SHH signaling (PubMed:19706761). GLI1 plays a role in cell proliferation and differentiation via its role in SHH signaling.