GLI2-Specific Polyklonaler Antikörper

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• KD/KO Validated

GLI2-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18989-1-PBS

Synonyme

Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2

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  WB analysis using 18989-1-AP (same clone as 18989-1-PBS)

  Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 18989-1-AP (same clone as 18989-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 18989-1-AP (same clone as 18989-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18989-1-AP (same clone as 18989-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18989-1-AP (GLI2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18989-1-AP (same clone as 18989-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18989-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA GLI2-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: GLI family zinc finger 2
• Berechnetes Molekulargewicht: 168 kDa
• Beobachtetes Molekulargewicht: 167 kDa， 88 kDa
• GenBank-Zugangsnummer: NM_005270
• Gene symbol: GLI2
• Gene ID (NCBI): 14633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.