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GLI4 Polyklonaler Antikörper

GLI4 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16551-1-AP

Synonyme

Zinc finger protein GLI4, Protein HKR4, Krueppel-related zinc finger protein 4, HKR4, GLI family zinc finger 4

Filter:
  • Western Blot (WB) analysis of Jurkat cells using GLI4 Polyclonal antibody (16551-1-AP)

    WB analysis of Jurkat using 16551-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GLI4 Polyclonal antibody (16551-1-AP)

    WB analysis using 16551-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using GLI4 Polyclonal antibody (16551-1-AP)

    IF Staining of A549 using 16551-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using GLI4 antibody (16551-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, PC-3-Zellen, SH-SY5Y-Zellen
Erfolgreiche Detektion in IF/ICCA549-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

16551-1-AP bindet in WB, IF/ICC, ELISA GLI4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GLI4 fusion protein Ag9497
Vollständiger Name GLI family zinc finger 4
Berechnetes Molekulargewicht 376 aa, 41 kDa
Beobachtetes Molekulargewicht 40-45 kDa
GenBank-ZugangsnummerBC014165
Gene symbol GLI4
Gene ID (NCBI) 2738
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for GLI4 antibody 16551-1-APProtokoll herunterladen
IF protocol for GLI4 antibody 16551-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

iru (Verified Customer) (12-14-2020)

It works at 1:500 O/N incubation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Fallopian tube epithelial cells