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GLIS3 Polyklonaler Antikörper

GLIS3 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12678-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of mouse kidney using 12678-1-AP (same clone as 12678-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of HepG2 using 12678-1-AP (same clone as 12678-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of A549 using 12678-1-AP (same clone as 12678-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using GLIS3 Polyclonal antibody (12678-1-AP)

    WB analysis of BxPC-3 using 12678-1-AP (same clone as 12678-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12678-1-AP (GLIS3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12678-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12678-1-PBS bindet in WB, Indirect ELISA GLIS3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen GLIS3 fusion protein Ag3366
Vollständiger Name GLIS family zinc finger 3
Berechnetes Molekulargewicht 775 aa, 84 kDa
Beobachtetes Molekulargewicht 70-85 kDa
GenBank-ZugangsnummerBC033899
Gene symbol GLIS3
Gene ID (NCBI) 169792
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. GLIS3 (gLIS family zinc finger 3), also known as ZNF515 that localizes to the nucleus and contains five C2H2-type zinc fingers. Expressed in a variety of tissues, including kidney, brain, liver, lung, ovary, pancreas, thymus and skeletal muscle, GLIS3 functions as both an activator and a suppressor of transcription, specifically binding the consensus sequence 5'-GACCACCCAC-3' through its C2H2-type zinc fingers. Defects in the gene encoding GLIS3 are a cause of NDH syndrome; a neonatal diabetes that is characterized by congenital hypothyroidism, congenital glaucoma, hepatic fibrosis and polycystic kidneys. There are various isoform of GLIS3 and molecular weight of one isoform is 70 kDa.