GLP1R Monoklonaler Antikörper

GLP1R Monoklonal Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A4G5

Kat-Nr. : 60931-1-Ig

Synonyme

GLP 1 R, GLP 1 receptor, GLP 1R, GLP-1 receptor, GLP-1R

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  IF Staining of rat pancreas using 60931-1-Ig

  FFPE-rat pancreas tissue was stained with GLP1R monoclonal antibody (Cat.NO. 60931-1-Ig) at 1:500 followed by detection with Multi-rAb® Polymer HRP-Goat Anti-Rabbit/Mouse Universal Recombinant Secondary Antibody (H+L) (Cat.NO. RGAU011). CoraLite®Plus 647-Tyramide Reagent (Cat.NO. PR30025) was used for signal development (purple). The slide was then conducted HIER in pH 9.0 Tris-EDTA buffer followed by staining with INS monoclonal antibody (66198-1-Ig, 1:5000). CoraLite®Plus 488-Tyramide Reagent (Cat.NO. PR30022) were used for signal development (green). Cell nucleus (blue) were stained with DAPI. The signal from each channel was captured and merged.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Ratten-Pankreasgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60931-1-Ig bindet in IF/ICC, ELISA GLP1R und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GLP1R fusion protein Ag23529
• Vollständiger Name: glucagon-like peptide 1 receptor
• Berechnetes Molekulargewicht: 463 aa, 53 kDa
• GenBank-Zugangsnummer: BC112126
• Gene symbol: GLP1R
• Gene ID (NCBI): 2740
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLP1R (glucagon-like peptide-1 receptor) is a G protein-coupled receptor belonging to the secretin receptor superfamily, also known as class B (PMID:34911028). GLP1R is widely distributed in pancreatic islets, muscles, the gastrointestinal tract, the lung, liver, pancreas, and other tissues or organs (PMID:35989517). Moreover, GLP1R agonist has been reported to induce autophagy of EC (Endometrial carcinoma) cells, and high GLP1R expression may be associated with good prognosis of EC patients, suggesting that GLP1R is likely to participate in the progression of EC (PMID:29907137). Moreover, regarding the molecular weight of GLP1R, research shows that the bands at ~50 kDa and ~100 kDa belong to the monomer and dimer states of GLP1R (PMID: 28609478).