GLRA2 Polyklonaler Antikörper

GLRA2 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13831-1-PBS

Synonyme

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  WB analysis of PBMC cells using 13831-1-AP (same clone as 13831-1-PBS)

  WB result of GLRA2 antibody (13831-1-AP, 1:600) with PBMC cells. Proteintech αGLRA2 13831-1-AP in 10% BSA / TBS 0.05% Tween-20 O/N @ 4°C Goat anti-rabbit HRP 1:10000 in 5% milk / TBS 0.05% Tween-20 1h @ RT. This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13831-1-AP (same clone as 13831-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13831-1-AP (GLRA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 13831-1-AP (same clone as 13831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 13831-1-AP (same clone as 13831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13831-1-AP (GLRA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13831-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13831-1-PBS bindet in WB, IHC, Indirect ELISA GLRA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRA2 fusion protein Ag4743
• Vollständiger Name: glycine receptor, alpha 2
• Berechnetes Molekulargewicht: 452 aa, 52 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC032864
• Gene symbol: GLRA2
• Gene ID (NCBI): 2742
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)