GLRA3 Polyklonaler Antikörper

GLRA3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 13145-1-AP

Synonyme

GLRA3, glycine receptor, alpha 3

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• 

  WB analysis of MCF-7 using 13145-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13145-1-AP (GLRA3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

13145-1-AP bindet in WB, ELISA GLRA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRA3 fusion protein Ag3702
• Vollständiger Name: glycine receptor, alpha 3
• Berechnetes Molekulargewicht: 464 aa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC036086
• Gene symbol: GLRA3
• Gene ID (NCBI): 8001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Publikationen

Species	Application	Title
mouse	WB	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
human	WB	Neuropharmacology; Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn.; Authors - Zi-Yang Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Braulio (Verified Customer) (02-26-2020)

Western blot in lumbar spinal cord samples, shows a mild band for alpha 3

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Spinal cord