GLRB Polyklonaler Antikörper

GLRB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 15371-1-AP

Synonyme

GLRB, Glycine receptor subunit beta, glycine receptor, beta

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  WB analysis of mouse brain using 15371-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 15371-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spinal cord using 15371-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15371-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Rückenmarkgewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Ratten-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

15371-1-AP bindet in WB, IHC, ELISA GLRB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRB fusion protein Ag4429
• Vollständiger Name: glycine receptor, beta
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC032635
• Gene symbol: GLRB
• Gene ID (NCBI): 2743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Publikationen

Species	Application	Title
mouse	WB	PLoS Biol; mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.; Authors - Zi-Yang Zhang
mouse	WB	Psychopharmacology (Berl); Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.; Authors - Niannian Huang
mouse	WB	J Neurosci; Spinal cord synaptic plasticity by GlyRβ release from receptor fields and syndapin I-dependent uptake; Authors - Jessica Tröger

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

A (Verified Customer) (11-11-2019)

Immunofluorescent analysis of (4% PFA) fixed rat DRG slices using GlyRB rabbit polyclonal antibody at 1:1000 dilution and Alexa 555 at 1:1000 dilution.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Rat DRG

GLRB Antibody Immunofluorescence, validation (1:1000 dilution) in Rat DRG (Cat no:15371-1-AP)