GLRX Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GLRX Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 15804-1-AP

Synonyme

Glutaredoxin 1, Glrx1, Glutaredoxin-1, Thioltransferase-1, TTase-1

---

• 

  WB analysis using 15804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15804-1-AP (GLRX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15804-1-AP (GLRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15804-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15804-1-AP (GLRX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 15804-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using GLRX antibody (15804-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IF: See 3 publications below

Produktinformation

15804-1-AP bindet in WB, IF/ICC, ELISA GLRX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRX fusion protein Ag8535
• Vollständiger Name: glutaredoxin (thioltransferase)
• Berechnetes Molekulargewicht: 106 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC005304
• Gene symbol: GLRX
• Gene ID (NCBI): 2745
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Elife; Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control; Authors - Meiling Wu
human	WB	Biochem Pharmacol; Ferroptosis induced by DCPS depletion diminishes hepatic metastasis in uveal melanoma; Authors - Bei Jin
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong ; KD Validated
human	WB	Sci Rep; Redox homeostasis protects mitochondria through accelerating ROS conversion to enhance hypoxia resistance in cancer cells.; Authors - Pengying Li
mouse	WB	J Bioenerg Biomembr; Glutaredoxin 1 protects neurons from oxygen-glucose deprivation/reoxygenation (OGD/R)-induced apoptosis and oxidative stress via the modulation of GSK-3β/Nrf2 signaling; Authors - Zhengguo Qiu ; KD Validated
human	WB	Mol Vis; Protective effect of Glutaredoxin 1 against oxidative stress in lens epithelial cells of age-related nuclear cataracts.; Authors - Qi Fan ; KD Validated