GLRX2 Polyklonaler Antikörper

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• KD/KO Validated

GLRX2 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13381-1-AP

Synonyme

GLRX2, Glutaredoxin 2, Glutaredoxin 2, mitochondrial, GRX2

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Galerie der Validierungsdaten

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  WB analysis using 13381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13381-1-AP (GLRX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 13381-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GLRX2 antibody (13381-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HGC-27 cells, MCF-7-Zellen
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below

Produktinformation

13381-1-AP bindet in WB, IF, ELISA GLRX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRX2 fusion protein Ag4218
• Vollständiger Name: glutaredoxin 2
• Berechnetes Molekulargewicht: 164 aa, 18 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC028113
• Gene symbol: GLRX2
• Gene ID (NCBI): 51022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLRX2(Glutaredoxin-2) is also named as GRX2 and belongs to the glutaredoxin family. It facilitates the maintenance of cellular redox homeostasis upon treatment with apoptotic agents, thereby preventing cardiolipin oxidation and cytochrome C release. The GLRX2, which exists in a dynamic equilibrium of enzymatically active monomers and quiescent dimers,is a 16-kDa protein directed either to the nucleus or to the mitochondria through differential splicing of the first exon(PMID: 17121859). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Free Radic Biol Med; Compartmentally scavenging hepatic oxidants through AMPK/SIRT3-PGC1α axis improves mitochondrial biogenesis and glucose catabolism.; Authors - Meiling Wu
human	WB	Free Radic Biol Med; Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.; Authors - Kelei Dong ; KD Validated
mouse	WB	Int Immunopharmacol; Glutaredoxin 2 protects cardiomyocytes from hypoxia/reoxygenation-induced injury by suppressing apoptosis, oxidative stress, and inflammation via enhancing Nrf2 signaling.; Authors - Chengliang Li
mouse	WB	Brain Res; Upregulation of Glutaredoxin 2 alleviates oxygen-glucose deprivation/reoxygenation-induced apoptosis and ROS production in neurons by enhancing Nrf2 signaling via modulation of GSK-3β.; Authors - Jian Wen ; KD Validated
mouse	WB	J Physiol Sci; Redox proteins are constitutively secreted by skeletal muscle.; Authors - Yasuko Manabe