GLRX3 Polyklonaler Antikörper

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• KD/KO Validated

GLRX3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 11254-1-AP

Synonyme

Glutaredoxin 3, GLRX4, Glutaredoxin-3, GRX3, GRX4

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  WB analysis using 11254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11254-1-AP (GLRX3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 11254-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11254-1-AP (GLRX3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 11254-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11254-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11254-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

11254-1-AP bindet in WB, IHC, ELISA GLRX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GLRX3 fusion protein Ag1775
• Vollständiger Name: glutaredoxin 3
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC014372
• Gene symbol: GLRX3
• Gene ID (NCBI): 10539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLRX3(Glutaredoxin-3) is also named as PICOT, TXNL2. Mammalian glutaredoxin 3 is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals(PMID:20226171). Glrx3 could take a pivotal role in colon and lung cancer cells during the tumorigenesis, which is expressed in lung and colon cancers consistently and preferentially(PMID:19797004).

Publikationen

Species	Application	Title
human	IHC	Chest; Proteomic analysis in lung tissue of smokers and COPD patients.; Authors - Lee Eun Joo EJ
human	WB	Hum Mol Genet; Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.; Authors - Ki Soon Kim
human	WB	Exp Cell Res; TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT.; Authors - Yi Zhao ; KD Validated
mouse	WB	Biosci Biotechnol Biochem; Identification of proteins that bind to the neuroprotective agent neoechinulin A.; Authors - Shinji Kamisuki ; KD Validated
human	WB	Haematologica; Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.; Authors - Nunziata Maio
human	WB	J Clin Invest; CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes; Authors - Nunziata Maio