GLRX3 Rekombinanter Antikörper

GLRX3 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251530F9

Kat-Nr. : 86587-1-PBS

Synonyme

Glutaredoxin 3, GLRX4, Glutaredoxin-3, GRX3, GRX4

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  WB analysis using 86587-1-RR (same clone as 86587-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86587-1-RR (GLRX3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86587-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02531-1

  The mean GLRX3 concentration was determined to be 669.5 ng/mL in HEK-293 cell extract based on a 1.1 mg/mL extract load, 358.1 ng/mL in A549 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02531-1

  Cytometric bead array standard curve of MP02531-1, GLRX3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86587-1-PBS. Detection antibody: 86587-2-PBS. Standard: Ag1775. Range: 1.562-100 ng/mL.

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  Affinity and Kinetic Characterization of 86587-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86587-1-RR against Human GLRX3 were performed. The affinity constant is 3.76 nM.

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Geprüfte Anwendungen

Produktinformation

86587-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA GLRX3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GLRX3 fusion protein Ag1775
• Vollständiger Name: glutaredoxin 3
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC014372
• Gene symbol: GLRX3
• Gene ID (NCBI): 10539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLRX3(Glutaredoxin-3) is also named as PICOT, TXNL2. Mammalian glutaredoxin 3 is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals(PMID:20226171). Glrx3 could take a pivotal role in colon and lung cancer cells during the tumorigenesis, which is expressed in lung and colon cancers consistently and preferentially(PMID:19797004).