GLUD1 Monoklonaler Antikörper

GLUD1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4G10D3

Kat-Nr. : 67026-1-PBS

Synonyme

GLUD, GDH1, GDH 1, GDH, EC:1.4.1.3

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  WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  WB analysis using 67026-1-Ig (same clone as 67026-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67026-1-Ig (GLUD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens) proteolytic pre-treatment mediated antigen retrieved with Tris-EDTA buffer (pH9). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67026-1-Ig (GLUD1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 67026-1-Ig (same clone as 67026-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using GLUD1 antibody (67026-1-Ig, Clone: 4G10D3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67026-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67026-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA GLUD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GLUD1 fusion protein Ag6179
• Vollständiger Name: glutamate dehydrogenase 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC040132
• Gene symbol: GLUD1
• Gene ID (NCBI): 2746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human glutamate dehydrogenase (GDH), an enzyme central to the metabolism of glutamate, is known to exist in housekeeping and nerve tissue-specific isoforms encoded by the GLUD1 and GLUD2 genes, respectively. It catalyses the reversible inter-conversion of glutamate to alpha-ketoglutarate and ammonia, thus interconnecting amino acid and carbohydrate metabolism. GLUD1 might contribute to the formation of specific synapses in the hippocampus such as those formed by the projecting neurons of the entorhinal cortex(PMID: 22138648). GLUD1 has a calculated molecular mass of 61 kDa and an apparent molecular mass of 45-55 kDa with the 53aa transit peptide removed.