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Glutamine Synthetase Polyklonaler Antikörper
Glutamine Synthetase Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF-P, FC (Intra), IP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11037-2-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, Jurkat-Zellen, Mauslebergewebe, Rattenlebergewebe |
Erfolgreiche IP | Mauslebergewebe |
Erfolgreiche Detektion in IHC | Mauslebergewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF-P | Mauslebergewebe, humanes Hirngewebe, Maus-Augengewebe |
Erfolgreiche Detektion in FC (Intra) | Jurkat-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:2000-1:16000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 3 publications below |
WB | See 30 publications below |
IHC | See 6 publications below |
IF | See 6 publications below |
IP | See 1 publications below |
ELISA | See 1 publications below |
Produktinformation
11037-2-AP bindet in WB, IHC, IF-P, FC (Intra), IP, ELISA Glutamine Synthetase und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | Glutamine Synthetase fusion protein Ag1510 |
Vollständiger Name | glutamate-ammonia ligase (glutamine synthetase) |
Berechnetes Molekulargewicht | 374 aa, 42 kDa |
Beobachtetes Molekulargewicht | 40-42 kDa |
GenBank-Zugangsnummer | BC011700 |
Gene symbol | Glutamine Synthetase |
Gene ID (NCBI) | 2752 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Glutamine Synthetase antibody 11037-2-AP | Protokoll herunterladen |
IHC protocol for Glutamine Synthetase antibody 11037-2-AP | Protokoll herunterladenl |
IF protocol for Glutamine Synthetase antibody 11037-2-AP | Protokoll herunterladen |
IP protocol for Glutamine Synthetase antibody 11037-2-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
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Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate
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Protein Cell Follicle stimulating hormone controls granulosa cell glutamine synthesis to regulate ovulation | ||
Sci Transl Med An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs. | ||
Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
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Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Marisa (Verified Customer) (07-29-2025) | I've used this antibody for immunohistochemistry in brain slices followed by confocal microscopy, and it performed consistently well. Clear signal, minimal background, and good lot-to-lot consistency. The datasheet and customer support were also helpful. Highly recommend for confocal applications.
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FH Andrea (Verified Customer) (09-06-2023) | antibody works perfect
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FH Melanie (Verified Customer) (02-02-2023) | Good antibody, which works well in HEK293T cells
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FH Ryan (Verified Customer) (02-27-2019) | Tris-HCL antigen retrieval ph=9.
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