GLUT1 Monoklonaler Antikörper

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• KD/KO Validated

GLUT1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2A5A2

Kat-Nr. : 66290-1-PBS

Synonyme

SLC2A1, SLC2A1,GLUT1, GLUT-1, 2A5A2

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  WB analysis of HEK-293 using 66290-1-Ig (same clone as 66290-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66290-1-Ig (SLC2A1,GLUT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66290-1-Ig (same clone as 66290-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66290-1-Ig (SLC2A1,GLUT1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66290-1-Ig (same clone as 66290-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66290-1-Ig (SLC2A1,GLUT1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66290-1-Ig (same clone as 66290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66290-1-Ig (same clone as 66290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66290-1-Ig (same clone as 66290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 66290-1-Ig (same clone as 66290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66290-1-Ig (same clone as 66290-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66290-1-Ig (GLUT1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 66290-1-Ig (same clone as 66290-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using GLUT1 antibody (66290-1-Ig, Clone: 2A5A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66290-1-Ig (same clone as 66290-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GLUT1 antibody (66290-1-Ig, Clone: 2A5A2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 66290-1-Ig (same clone as 66290-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human GLUT1 (66290-1-Ig, Clone:2A5A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66290-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66290-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA GLUT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GLUT1 fusion protein Ag17108
• Vollständiger Name: solute carrier family 2 (facilitated glucose transporter), member 1
• Berechnetes Molekulargewicht: 492 aa, 54 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC121804
• Gene symbol: GLUT1
• Gene ID (NCBI): 6513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glucose transporter 1 (GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein responsible for the transport of glucose in many cell types and across the blood-brain barrier.

What is the molecular weight of GLUT1? Is GLUT1 post-translationally modified?

There are two forms of GLUT1 transporter that differ in their molecular weight. The 45-kDa form is found in glial cells, while the 55-kDa form is present in the endothelial cells regulating glucose transport over the blood-brain and blood-tissue barriers (PMID: 9630522). N-glycosylation of asparagine at position 42 is the only known post-translation modification of GLUT1 (PMID: 3839598).

What is the subcellular localization of GLUT1?

Glucose transporters, including GLUT1, are multiple-pass integral membrane proteins. GLUT1 is present at the plasma membrane but is also a subject of recycling between plasma membrane and endosomes.

What molecules can be transported by GLUT1?

The main substrate of GLUT1 transport is glucose, but it can also transport galactose, mannose, glucosamine, and reduced ascorbate. 

What is the tissue expression pattern of GLUT1?

GLUT1 is expressed by many cell types but the highest levels are observed in erythrocytes and in the central nervous system (astrocytes). GLUT1 is responsible for glucose transfer across the blood-brain and blood-tissue barriers, including placental transport.