GLUT1 Rekombinanter Antikörper

GLUT1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6F14

Kat-Nr. : 81463-1-PBS

Synonyme

SLC2A1, 6F14, GLUT-1, SLC2A1,GLUT1

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  WB analysis using 81463-1-RR (same clone as 81463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81463-1-RR (SLC2A1,GLUT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 81463-1-RR (same clone as 81463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 81463-1-RR (same clone as 81463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 81463-1-RR (GLUT1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81463-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81463-1-PBS bindet in WB, IHC, Indirect ELISA GLUT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GLUT1 fusion protein Ag16282
• Vollständiger Name: solute carrier family 2 (facilitated glucose transporter), member 1
• Berechnetes Molekulargewicht: 492 aa, 54 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC121804
• Gene symbol: GLUT1
• Gene ID (NCBI): 6513
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUT1, also known as SLC2A1, is a ubiquitously expressed glucose transporter and is responsible for the basal level of glucose uptake in most cell types. Human erythrocytes express the highest level of GLUT1. Defects in SLC2A1 are the cause of GLUT1 deficiency syndrome type 1 and type 2. High expression of GLUT1 has been reported to be a reliable immunohistochemical marker for juvenile hemangiomas. GLUT1 protein may appear as two or more distinct forms among 43 kDa to 55 kDa due to the different glycosylation states. And the conversion of the highly glycosylated form of GLUT1 to a less glycosylated form has been reported to correlate with differentiation (PMID: 8263524, 23302780).