GLUT2 Monoklonaler Antikörper

GLUT2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G8B1

Kat-Nr. : 66889-1-PBS

Synonyme

GLUT 2, GLUT2, SLC2A2

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  WB analysis of 4T1 using 66889-1-Ig (same clone as 66889-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66889-1-Ig (same clone as 66889-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66889-1-Ig (same clone as 66889-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66889-1-Ig (same clone as 66889-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66889-1-Ig (SLC2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66889-1-Ig (same clone as 66889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66889-1-Ig (same clone as 66889-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66889-1-Ig (GLUT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66889-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66889-1-PBS bindet in WB, IHC, Indirect ELISA GLUT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: GLUT2 fusion protein Ag14551
• Vollständiger Name: solute carrier family 2 (facilitated glucose transporter), member 2
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC060041
• Gene symbol: GLUT2
• Gene ID (NCBI): 6514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUT2 is a member of glucose transporters or GLUTs which catalyze the glucose transport across plasma membrane. GLUT2 is selectively expressed in hepatocytes, pancreatic beta cells, and absorptive renal and intestinal epithelial cells. GLUT2 is required to maintain normal glucose homeostasis and normal function and development of the endocrine pancreas. Its absence leads to symptoms characteristic of non-insulin-dependent diabetes mellitus.