GM-CSF Rekombinanter Antikörper

GM-CSF Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240183C7

Kat-Nr. : 98050-1-PBS

Synonyme

CSF2, CSF 2, CSF, Colony-stimulating factor, Colony Stimulating Factor 2

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  FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

  1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98050-1-RR (same clone as 98050-1-PBS)

  1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human GM-CSF Rabbit Recombinant Antibody (98050-1-RR, Clone:240183C7) and PE-conjugated Goat Anti-Rabbit IgG(red). 1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98050-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98050-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98050-1-RR against Human GM-CSF were performed. The affinity constant is 3.46 nM.

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Geprüfte Anwendungen

Produktinformation

98050-1-PBS bindet in FC (Intra) GM-CSF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: colony stimulating factor 2 (granulocyte-macrophage)
• Gene symbol: GM-CSF
• Gene ID (NCBI): 1437
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.