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GM-CSF Rekombinanter Antikörper

GM-CSF Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

243117B1

Kat-Nr. : 98463-3-PBS

Synonyme

Colony-stimulating factor, CSF, Csf2, Csfgm, Granulocyte-macrophage colony-stimulating factor

Filter:
  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Rat GM-CSF Rabbit Recombinant Antibody (98463-3-RR)

    FC experiment of Transfected HEK-293T using 98463-3-RR (same clone as 98463-3-PBS)

    1x10^6 HEK-293T cells or GM-CSF transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat GM-CSF Rabbit RecAb (98463-3-RR, Clone: 243117B1) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98463-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using Anti-Rat GM-CSF Rabbit Recombinant Antibody (98463-3-RR)

    FC experiment of Transfected HEK-293T using 98463-3-RR (same clone as 98463-3-PBS)

    1x10^6 HEK-293T cells or GM-CSF transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Rat GM-CSF/CSF2 Rabbit RecAb (98463-3-RR, Clone: 243117B1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98463-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

98463-3-PBS bindet in FC (Intra) GM-CSF und zeigt Reaktivität mit Ratten

Getestete Reaktivität Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen GM-CSF fusion protein Eg3202
Vollständiger Name colony stimulating factor 2 (granulocyte-macrophage)
Berechnetes Molekulargewicht17 kDa
GenBank-ZugangsnummerNM_053852.1
Gene symbol Csf2
Gene ID (NCBI) 116630
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C.

Hintergrundinformationen

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.