GMAP-210 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GMAP-210 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (2)

Kat-Nr. : 26456-1-AP

Synonyme

TRIP11, TR-interacting protein 11, Thyroid receptor-interacting protein 11, Golgi-associated microtubule-binding protein 210, GMAP 210

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  WB analysis of HeLa using 26456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26456-1-AP (GMAP-210 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 26456-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26456-1-AP (GMAP-210 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 26456-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 26456-1-AP (GMAP-210 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human thyroid cancer using 26456-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26456-1-AP (GMAP-210 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 26456-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 26456-1-AP (GMAP-210 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26456-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26456-1-AP (GMAP-210 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 26456-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GMAP-210 antibody (26456-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HL-60-Zellen
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

26456-1-AP bindet in WB, IHC, IF/ICC, ELISA GMAP-210 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GMAP-210 fusion protein Ag13867
• Vollständiger Name: thyroid hormone receptor interactor 11
• Berechnetes Molekulargewicht: 1979 aa, 228 kDa
• Beobachtetes Molekulargewicht: 230 kDa
• GenBank-Zugangsnummer: BC002656
• Gene symbol: TRIP11
• Gene ID (NCBI): 9321
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Golgi microtubule-associated protein 210 (GMAP-210), also referred to as CEV14, Trip11 or Trip230, is a peripheral Golgi protein that localizes to the cis-Golgi network. GMAP-210 is a 1,978 amino acid coiled-coil member of the golgin family of proteins. Microtubule ends bind to GMAP-210 which functions to link the cis-Golgi network to the minus ends of centrosome-nucleated microtubules. This interaction may be essential for the proper morphology and structural maintenance of the Golgi apparatus. GMAP-210 also associates with thyroid hormone receptor. Overexpression of GMAP-210 disrupts the micro-tubule network and causes a significant enlargement and fragmentation of the Golgi apparatus; it also blocks anterograde and retrograde transport between the ER and the Golgi apparatus.

Publikationen

Species	Application	Title
human	IF	J Biol Chem; Tumor protein D54 binds intracellular nanovesicles via an extended amphipathic region.; Authors - Antoine Reynaud
human	WB	Commun Biol; Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.; Authors - Xihua Yue
human	WB,IF	Hum Mutat; Biallelic deep intronic variant c.5457+81T>A in TRIP11 causes loss of function and results in achondrogenesis 1A.; Authors - Priyanka Upadhyai
human	WB	Biol Open; GRASP55 restricts early-stage autophagy and regulates spatial organization of the early secretory network.; Authors - Jennifer Y Liu ; KD Validated
		Nat Commun; Dynamic movement of the Golgi unit and its glycosylation enzyme zones; Authors - Akihiro Harada

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

• Applications: Western Blot

FH

Yuki (Verified Customer) (01-24-2023)

I will recommend this antibody for IF

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: 293T

GMAP-210 Antibody Immunofluorescence validation (1:250 dilution) in 293T (Cat no:26456-1-AP)